Genome Sequencing

-

What is Genome sequencing?

A Genome contains the entire genetic information (genetic code)  of an organism. Sequencing, it is a way of decoding this information. for example decoding of a viral genome can help us understand how it replicates and mutates.

Genome sequencing is a technique that involves analysing the genetic code of an organism and is done with the help of powerful computers. For instance to find out any possible presence a mutation of sars-Cov-2- the virus that causes Covid-19- and understand its role in the infection's transmission and spread. 

RNA (Ribonucleic acid ) it translates genetic material to protein. RNA is extracted and converted to complementary Dioxyribonucleic acid (cDNA). This in turn was amplified and cut into smaller pieces ( a process called fragmenting) since the sequencer is designed for a very specific size of DNA fragments. These were tagged with linker sequences (to help link the sample for further steps like barcoding). Each sample is barcoded( given a unique identification) and then pooled together.

Uses

it is used for following purposes:

  1. Disease forecasting 
  2. Gene repairing
  3. Gene analysis

Comments

Post a Comment

Popular posts from this blog

ALGAL BLOOM?

-
Image
 ALGAL BLOOM   Keywords :  Alga (singular subject/ noun) Algae ( plural subject/noun) Mycrocystis (alga) microcystin( toxin / poison) DO: oxygen dissolved in water, generally 4mg/l required to sustain the life in water.  Eutrophication   (Neutrients enrichment in water bodies ) : The excess of fixed nitrogen builds up in streams and lakes where provides extra food for microorganisms and causes a bloom in the population of algae. The algae population eat up oxygen and eventually, brings the waterbody on the brinks of death. Algal bloom in river  Algal bloom( algal pollution) is a condition of excessive sudden  population growth of algae on the surface of water bodies in mat form because of sudden increase of nutrients like nitrogen and phosphorus that leads food chain disturbance in water bodies. Actually microscopic plants( algae) are unicellular, aquatic, roots, and leaves. Algae are required nutrients for growth by the process of photosynthesis; ...
Read more

Sati

-
  Topic.        Indian society .        Modern history   Issues related to women during colonial period  Prelims  Mains GS1  Sati   It was a orthodox evil practice to burn widow alive on her husband's pyre without her will. This practice was largely followed by the higher classes of the society.  It was William bentinck who made it a crime against humanity in 1829 and strongly supported by  Rajaram Mohan Ray. It was the main achievement of British government in India to to modernise the societies of India  Government of India passed an act in 1856 to enable widow remarriage  and  Pandit Iswar Chandra Vidya Sagar had requested for making law regarding widow remarriage.  Government of India tried to practice of killing women infanticide and dowry system.
Read more

Zika Virus

-
Topic.                Health diseases                                                             Prelims   Zika virus Zika virus disease is caused by a virus transmitted primarily by Aedes Mosquitoes and Albopictus Mosquitoes, which bite during the day.  Zika is Mosquitoes borne virus that spread through the bite of an infected  Aedes species of Mosquitoes named Aedes Aegypti and Albopictus Mosquitoes by a virus called Zika.  Zika virus is caused by the Same Mosquito that causes Dengue. According to the WHO the Aedes Mosquitoes usually bite during the day, peaking during early morning and late afternoon or evening.  What is mean by Mosquitoes borne virus? Borne means carried by or moved by a particular thing such as airborne diseases in which diseases is carried by one pla...
Read more